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Image Search Results
Journal: Archives of Toxicology
Article Title: Mouse innate-like B-1 lymphocytes promote inhaled particle-induced in vitro granuloma formation and inflammation in conjunction with macrophages
doi: 10.1007/s00204-021-03200-2
Figure Lengend Snippet: Late and specific accumulation of lung B-1 lymphocytes after silica treatment. a Gating strategy in flow cytometry of B-2 lymphocytes (CD23 + ) and B-1 lymphocytes (CD23 − ) from CD45 + CD19 + CD22 + cells or plasma cells (CD45 + CD19 − CD138 + ) in the lung; representative images of the populations obtained from mouse lungs 15 days after instillation of NaCl. b , c Proportions ( b ) and numbers ( c ) of B-1 lymphocytes, B-2 lymphocytes and plasma cells in the lungs of control mice and of mice treated with silica (1, 3, 7, 15, 30 and 60 days after instillation of SiO 2 , n = 5 per time, 2.5 mg/mouse). The control column (CTRL) shows the combined results obtained from control mice analyzed at each studied time point ( n = 14, no significant difference between control groups, see “ ”)
Article Snippet: Cells were incubated with Fc block (anti-CD16 and anti-CD32; BD Biosciences, Franklin Lakes, New Jersey, USA) and different fluorochrome-coupled antibodies as follows: anti-CD45 (clone 30-F11; FITC; BD Biosciences), anti-CD5 (clone 53-7.3; PE and PE-Vio770; Miltenyi Biotec), anti-CD11b (clone REA592, Vioblue; Miltenyi Biotec), anti-CD22 (clone Cy34.1; Pe-Vio770; Miltenyi Biotec), anti-CD23 (clone B3B4; APC; Miltenyi Biotec), anti-CD38 (clone 90.4; APC; Miltenyi Biotec), anti-CD43 (clone REA840; PE; Miltenyi Biotec) and anti-CD138 (clone REA104; PE; Miltenyi Biotec),
Techniques: Flow Cytometry, Clinical Proteomics, Control
Journal: Archives of Toxicology
Article Title: Mouse innate-like B-1 lymphocytes promote inhaled particle-induced in vitro granuloma formation and inflammation in conjunction with macrophages
doi: 10.1007/s00204-021-03200-2
Figure Lengend Snippet: B-1a and B-1b subpopulations accumulate in silica-treated lungs during fibrotic granuloma formation. a Gating strategy in flow cytometry of B-2 lymphocytes (CD23 + ), B-1a cells (CD23 − CD5 + ) and B-1b lymphocytes (CD23 − CD5 − ) from CD45 + CD19 + CD22 + cells; representative images of the populations obtained from mouse lungs 15 days after instillation of NaCl. b Numbers of B-1a and B-1b lymphocytes in the lungs of control mice and of mice treated with silica (1, 3, 7, 15, 30 and 60 days after instillation of SiO 2 , n = 5 per time, 2.5 mg/mouse). The control column (CTRL) shows the combined results obtained from control mice analyzed at each studied time point ( n = 14, no significant difference between control groups, see “ ”). c Numbers of pulmonary B-2, B-1a and B-1b lymphocytes in the control group (CTRL) or mice treated with tungsten carbide (WC, n = 3, 2.5 mg/mouse), silica (SiO 2 , n = 5, 2.5 mg/mouse) or carbon nanotubes (CNT, n = 4, 0.2 mg/mouse) 60 days after instillation
Article Snippet: Cells were incubated with Fc block (anti-CD16 and anti-CD32; BD Biosciences, Franklin Lakes, New Jersey, USA) and different fluorochrome-coupled antibodies as follows: anti-CD45 (clone 30-F11; FITC; BD Biosciences), anti-CD5 (clone 53-7.3; PE and PE-Vio770; Miltenyi Biotec), anti-CD11b (clone REA592, Vioblue; Miltenyi Biotec), anti-CD22 (clone Cy34.1; Pe-Vio770; Miltenyi Biotec), anti-CD23 (clone B3B4; APC; Miltenyi Biotec), anti-CD38 (clone 90.4; APC; Miltenyi Biotec), anti-CD43 (clone REA840; PE; Miltenyi Biotec) and anti-CD138 (clone REA104; PE; Miltenyi Biotec),
Techniques: Flow Cytometry, Control